Peer-reviewed Publications
Our scientific team is published in peer-reviewed journals worldwide.
Coales SJ, E SY, Lee JF, Ma A, Morrow JA, Hamuro Y. Expansion of time window for mass spectrometric measurement of amide hydrogen/deuterium exchange reactions. Rapid Commun Mass Sp. 2010;24:3584-3592.
Lewis HA, Wang C, Zhao X, Hamuro Y, Conners K, Kearins MC, et al. Structure and dynamics of NBD1 from CFTR characterized using crystallography and hydrogen-deuterium exchange mass spectrometry. J Mol Biol. 2010;396:406-430.
Gerhardt S, Abbott WM, Hargreaves D, Pauptit RA, Davies RA, Needham MR, et al. Structure of IL-17A in complex with a potent, fully human neutralizing antibody. J Mol Biol. 2009;394(5):905-21. Epub 2009 Oct 14.
Molnar KS, Wang Q, Karabacak NM, Tiwari A, Hayward LJ, Coales SJ, et al. A common property of amyotrophic lateral sclerosis-associated variants: Destabilization of the Cu/Zn superoxide dismutase electrostatic loop. J Biol Chem. 2009;284:30965-30973.
Maegawa GH, Tropak MB, Buttner JD, Rigat BA, Fuller M, Pandit D, et al. Identification and characterization of Ambroxol as an enzyme enhancement agent for Gaucher disease. J Biol Chem. 2009;284:23502-23516.
Sharma S, Zheng H, Huang YJ, Ertekin A, Hamuro Y, Rossi P, et al. Construct optimization for protein NMR structure analysis using amide hydrogen/deuterium exchange mass spectrometry. Proteins. 2009;76:882-894.
Coales SJ, Tuske SJ, Tomasso JC, Hamuro Y. Epitope mapping by amide hydrogen/deuterium exchange coupled with proteolysis and liquid chromatography mass spectrometry. Rapid Commun Mass Sp. 2009;23(5):639-647.
Chandra V, Huang P, Hamuro Y, Raghuram S, Wang Y, Burris TP, et al. Structural organization of the intact PPARγ-RXRα nuclear receptor complex on DNA. Nature. 2008;456(7220):350-356.
Tropak MB, Kornhaber G, Rigat B, Maegawa G, Buttner J, Blanchard J, et al. Identification of pharmacological chaperones for Gaucher disease and characterization of their effects on b-Glucocerebrosidase by hydrogen/deuterium exchange mass spectrometry. ChemBioChem. 2008;9(16):2650-2662.
Kornhaber GJ, Tropak MB, Maegawa G, Tuske SJ, Coales SJ, Mahuran DJ, et al. Isofagomine induced stabilization of glucocerebrosidase. ChemBioChem. 2008;9(16):2643-2649.
Hamuro Y, Tomasso JC, Coales SJ. A simple test to detect hydrogen/deuterium scrambling during gas phase peptide fragmentation. Anal Chem. 2008;80(17):6785-6790. Epub 2008 Jul 31.
Hamuro Y, Hamuro LL, Coales SJ, Woods VL Jr. Use of enhanced peptide amide hydrogen/deuterium exchange-mass spectrometry (DXMS) in the examination of protein-protein interactions. In: Chance M, ed. Mass Spectrometry Analysis for Protein-Protein Interactions and Dynamics. In press.
Luo L, Carson JD, Molnar KS, Tuske SJ, Coales SJ, Hamuro Y, et al. Conformation-dependent ligand regulation of ATP hydrolysis by human KSP: Activation of basal hydrolysis and inhibition of microtubule-stimulated hydrolysis by a single, small molecule modulator. J Am Chem Soc. 2008;130,7584-7591.
Schuster MC, Papp K, Molnar KS, Coales SJ, Hamuro Y, Sfyroera G, et al. Dynamic structural changes during complement C3 activation analyzed by hydrogen/deuterium exchange mass spectrometry. Mol Immunol. 2008;45:3142-3151.
Coales SJ, Tomasso JC, Hamuro Y. Effects of electrospray capillary temperature on amide hydrogen exchange. Rapid Commun Mass Sp. 2008;22:1367-1371.
Hamuro Y, Coales SJ, Molnar KS, Tuske SJ, Morrow JA. Specificity of immobilized porcine pepsin in H/D exchange compatible conditions. Rapid Commun Mass Sp. 2008;22:1041-1046.
Hamuro Y, Molnar KS, Coales SJ, Yang BO, Simorellis A, Pochapsky TC. Hydrogen-deuterium exchange spectrometry for investigation of backbone dynamics of oxidized and reduced cytochrome P450cam. J Inorg Biochem. 2008;102:364.
Hamuro Y, Coales SJ, Woods VL Jr. Protein-targeting drug discovery guided by hydrogen/deuterium exchange mass spectrometry (DXMS). In Wanner KT, ed. Mass Spectrometry in Medicinal Chemistry. Wiley-VCH; 2007.
Hamuro Y, Coales SJ, Morrow JA, Griffin PR, Southern MR, Weber PC. Application of hydrogen/deuterium-exchange to p38 mitogen-activated protein kinase. Am Biotechnol Lab. 2007:28.
Horn JR, Kraybill B, Petro EJ, Coales SJ, Morrow JA, Hamuro Y, et al. The role of protein dynamics in increasing the binding affinity of an engineered protein-protein interaction established by H/D exchange mass spectrometry. Biochemistry. 2006;45: 8488-8498.
Hamuro Y, Coales SJ, Morrow JA, Molnar KS, Tuske SJ, Southern MR, et al. Hydrogen/deuterium-exchange (H/D-Ex) of PPARγ LBD in the presence of various modulators. Protein Sci. 2006;15:1-10.
Yu B, Edstrom WC, Hamuro Y, Weber PC, Gibney BR, Hunt J. Crystal structure of catalytic complex of the Fe(II)-oxoglutarate-dependent DNA repair enzyme AlkB give insight into promiscuous substrate recognition and oxidation chemistry. Nature. 2006; 439:879-884.
Hamuro Y (co-first authored), Burns-Hamuro LL, Kim JS, Sigala P, Fayos R, Stranz DD, et al. Distinct interaction modes for an AKAP bound to two regulatory subunit isoforms of PKA revealed by amide hydrogen/deuterium exchange. Protein Sci. 2005;14:2982-2992.
Hamuro Y, Weber PC, Griffin PR. High throughput analysis of protein structure by hydrogen/deuterium exchange mass spectrometry. In Figeys D, ed. Industrial Proteomics. NY: Wiley; 2005.
Hamuro Y, Anand GS, Kim JS, Juliano C, Stranz DD, Taylor SS, et al. Mapping intersubunit interactions of the regulatory subunit (RIα) in the Type I holoenzyme of protein kinase A by enhanced amide hydrogen/deuterium exchange mass spectrometry (DXMS). J Mol Biol. 2004;340:1185-1196.
Hamuro Y, Coales SJ, Southern MR, Nemeth-Cawley JF, Stranz DD, Griffin PR. Rapid analysis of protein structure and dynamics by hydrogen/deuterium exchange mass spectrometry. J Biomol Tech. 2003;14:171-182.
Zawadzki KM, Hamuro Y, Kim JS, Garrod S, Stranz DD, Taylor SS. Dissecting interdomain communication within cAPK regulatory subunit type IIβ using enhanced amide hydrogen/deuterium exchange mass spectrometry (DXMS). Protein Sci. 2003;12:1980-1990.
Englander JJ, Delmar C, Li W, Englander SW, Kim JS, Stranz DD, et al. Protein structure change studied by hydrogen-deuterium exchange, functional labeling, and mass spectrometry. P Natl Acad Sci USA. 2003;100:7057-7062.
Hamuro Y, Zawadzki KM, Kim JS, Stranz DD, Taylor SS, Woods VL Jr. Dynamics of cAPK type II? activation revealed by enhanced amide H/2H exchange mass spectrometry (DXMS). J Mol Biol. 2003;327:1065-1076.
Hamuro Y, Wong L, Shaffer J, Kim JS, Stranz DD, Jennings PA, et al. Phosphorylation-driven motions in the COOH-terminal Src kinase, Csk, revealed through enhanced Hydrogen-Deuterium Exchange and Mass Spectrometric Studies (DXMS). J Mol Biol. 2002;323:871-881.
Hamuro Y, Burns LL, Canaves JM, Hoffman RC, Taylor SS, Woods VL Jr. Domain organization of D-AKAP2 revealed by enhanced deuterium exchange-mass spectrometry (DXMS). J Mol Biol. 2002;321:703-714.
Woods VL Jr, Hamuro Y. High resolution, high-throughput amide deuterium exchange-mass spectrometry (DXMS) determination of protein binding site structure and dynamics: Utility in pharmaceutical design. J Cell Biochem. 2002;37(suppl):89-98.